Time-dependent involvement of PKA/PKC in contextual memory consolidation
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Updated: Sep 12, 2026

Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons
Published on: April 6, 2011
Gene V Wallenstein1, David R Vago, Anne M Walberer
1Department of Psychology, Cognitive Neurobiology Institute, University of Utah, Salt Lake City 84112, USA. cogneuro_institute@hotmail.com
Abstract:
Rats were implanted bilaterally with cannulae into the dorsal hippocampus and trained in a Pavlovian fear-conditioning paradigm. Four groups of rats were infused intra-cranially with 1-(5'-isoquinolinesulfonyl)-2-methylpiperazine (H7-dihydrochloride), a potent inhibitor of both protein kinase C (PKC) and cAMP-dependent protein kinase (PKA), at different time intervals in order to examine their involvement in the acquisition and consolidation of contextual fear memory. We demonstrate a significant consolidation deficit of long-term contextual fear-conditioning memory that is maximal when PKA and PKC are inhibited at 90 min post-training. These results suggest the existence of a critical time window, during which these enzymes must be activated for the consolidation of long-term memories.
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