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Updated: Aug 15, 2026

Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example
Published on: October 8, 2014
Osmosis without solute
Abstract:
Van't Hoff's misconception of the kinetics of solutes in a solution was long ago displaced by the thesis about osmotic water migration caused by the difference in chemical potential on the two sides of the semipermeable membrane. Consequently the osmotic process was now investigated when normal water and heavy water were separated by the "semipermeable" membrane. Due to the difference between the chemical potentials a substantial osomotic pressure was measured on the side of the heavy water simultaneously with a significant migration of water on both sides.
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