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Altered cell wall disassembly during ripening of Cnr tomato fruit: implications for cell adhesion and fruit

Caroline Orfila1, Miranda M H Huisman, William G T Willats

  • 1Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT, UK.

Planta
|July 12, 2002
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Summary
This summary is machine-generated.

The non-ripening Cnr tomato mutant shows altered fruit ripening due to changes in cell wall pectic polysaccharides. These changes impact fruit softening and cell adhesion, offering insights into tomato fruit development.

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Area of Science:

  • Plant Biology
  • Fruit Ripening Physiology
  • Molecular Genetics

Background:

  • The Cnr (Colourless non-ripening) tomato mutant exhibits impaired fruit ripening, characterized by a lack of softening and reduced pericarp cell adhesion.
  • Understanding cell wall modifications, particularly pectic polysaccharides, is crucial for elucidating the Cnr phenotype.

Purpose of the Study:

  • To investigate the role of pectic polysaccharides in the aberrant fruit ripening and altered cell adhesion of Cnr tomatoes.
  • To analyze cell wall composition and extractable fractions in Cnr and wild-type tomatoes during ripening.

Main Methods:

  • Analysis of cell wall material (CWM) and solubilized fractions from mature green and red ripe Cnr and wild-type tomatoes.
  • Utilized chemical, enzymatic, and immunochemical techniques to characterize pectic polysaccharides.
  • Assessed the solubility and degradation susceptibility of cell wall components.

Main Results:

  • No significant differences in overall CWM sugar composition were observed between Cnr and wild-type.
  • Ripening-associated solubilization of homogalacturonan-rich pectic polysaccharides was reduced in Cnr fruit.
  • Cnr cell walls showed decreased chelator-soluble carbohydrates and increased tightly bound, insoluble polysaccharides.

Conclusions:

  • Altered pectic polysaccharide profiles, particularly reduced homogalacturonan solubilization and increased insoluble fractions, contribute to the non-softening and altered cell adhesion phenotype in Cnr tomatoes.
  • These findings highlight the complex changes in cell wall polymers during fruit ripening and their significance for tomato fruit development.