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An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells

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Published on: July 20, 2014

Leg patterning driven by proximal-distal interactions and EGFR signaling.

M I Galindo¹, S A Bishop, S Greig

¹School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK.

Science (New York, N.Y.)

|July 13, 2002

Summary

Wingless and decapentaplegic signaling are not essential for all Drosophila leg development. Distal leg structures, like the tarsus, are patterned by Distalless, dachshund, and epidermal growth factor receptor-Ras signaling.

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Area of Science:

Developmental Biology
Genetics
Evolutionary Biology

Background:

The proximal-distal axis of Drosophila legs is established by wingless and decapentaplegic signaling pathways.
These pathways activate key genes like Distalless and dachshund in broad domains during early leg development.

Purpose of the Study:

To investigate the role of wingless and decapentaplegic signaling throughout proximal-distal leg development.
To identify the signaling mechanisms that define the distal leg segment, specifically the tarsus.

Main Methods:

Genetic analysis of gene expression patterns in Drosophila melanogaster.
Perturbation of wingless, decapentaplegic, Distalless, dachshund, and epidermal growth factor receptor (EGFR)-Ras signaling pathways.

Main Results:

Wingless and decapentaplegic signaling are not required for the entire duration of proximal-distal leg development.
The tarsus is defined by the activity of Distalless, dachshund, and a distal gradient of EGFR-Ras signaling.
This contrasts with the broader roles of wingless and decapentaplegic in early leg patterning.

Conclusions:

A novel mechanism for appendage patterning involves genes acting in specific proximal-distal domains.
The findings suggest a conserved mechanism for appendage patterning across arthropods and vertebrates.
The tarsus's definition relies on a distinct set of signaling interactions compared to earlier developmental stages.