Method for analysis of 4-hydroxy-2-(E)-nonenal with solid-phase microextraction
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Updated: Sep 2, 2026
A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples
Published on: July 16, 2017
Tatsuhiro Uchida1, Naohiro Gotoh, Shun Wada
1Department of Food Science and Technology, Tokyo University of Fisheries, Japan.
Abstract:
A simple analytical method for 4-hydroxy-2-(E)-nonenal (HNE) using solid-phase microextraction (SPME) fiber was developed. HNE or the derivative of HNE formed by reaction with 2,4-dinitrophenylhydrazine (DNPH) was extracted from the sample solution by immersing the SPME fiber into the solution, and the amount of HNE was quantified by HPLC. The extraction conditions of HNE and HNE-DNPH were examined, using standard solutions, with respect to fiber coating, NaCl concentration, rate of stirring, adsorption temperature, and adsorption time. The recovery of HNE reached 80%, and the quantification limits of HNE and HNE-DNPH using standard compounds were 14.1 pmol/10 mL and 486.5 fmol/10 mL, respectively. This method can be applied to the detection of HNE in oxidized oil or samples of porcine liver.
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