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Artificial regulation of transcription applying carbohydrate-lectin interaction
Kazunori Matsuura1, Katsuhiro Hayashi, Kazukiyo Kobayashi
1Department of Molecular Design, Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya 464-8603, Japan.
Chemical Communications (Cambridge, England)
|July 19, 2002
Abstract:
A complex of plasmid DNA-lactose conjugate with lectin is proposed as an artificial system to control transcription activity: the in vitro transcription of DNA in the conjugate with T7 RNA polymerase was repressed in the presence of RCA120, and then the transcription ability was recovered by adding lactose or a lactose-carrying polymer to the repression system.