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Molecular characterization of three newly recognized rat parvoviruses
Cho-Hua Wan1, Maria Söderlund-Venermo1, David J Pintel1
1Department of Veterinary Pathobiology1 and Department of Molecular Microbiology & Immunology2, University of Missouri, Columbia, MO 65211, USA.
Abstract:
Rodent parvoviruses have been documented to interfere with both in vivo and in vitro research. In this study, three rat parvoviruses distinct from previously characterized rodent parvoviruses were identified from naturally infected rats obtained from four discrete sources. These three newly recognized parvoviruses were designated rat minute virus (RMV)-1a, -1b and -1c. In this study, the genomic nucleotide sequence and the predicted amino acid sequences of proteins for each of the three RMV-1 variants and Kilham rat virus (KRV) were determined and compared with previously characterized rodent parvoviruses. The three RMV-1 variants were shown to be closely related to each other, to be distinct from but closely related to KRV and H-1 virus, and to be significantly different from the previously identified rat parvovirus isolate, RPV-1a.
Insights
Three new rat parvoviruses, designated RMV-1a, -1b, and -1c, were identified. These viruses are distinct from known rodent parvoviruses and closely related to Kilham rat virus (KRV).
Area of Science:
- Virology
- Molecular Biology
- Infectious Diseases
Background:
- Rodent parvoviruses can significantly impact the reliability of biological research.
- Accurate identification and characterization of novel viral agents are crucial for understanding their potential effects.
Purpose of the Study:
- To identify and characterize novel rat parvoviruses.
- To compare the genetic sequences of new isolates with known rodent parvoviruses.
Main Methods:
- Isolation and identification of parvoviruses from naturally infected rat populations.
- Genomic nucleotide sequencing of novel rat minute virus (RMV)-1 variants and Kilham rat virus (KRV).
- Bioinformatic analysis and comparison of viral protein sequences.
Main Results:
- Three distinct RMV-1 variants (RMV-1a, -1b, -1c) were identified.
- These RMV-1 variants are genetically closely related to each other.
- RMV-1 variants are distinct from, but closely related to, KRV and H-1 virus, and significantly different from RPV-1a.
Conclusions:
- Discovery of three novel rat parvovirus variants, RMV-1a, -1b, and -1c.
- These findings expand the known diversity of rodent parvoviruses.
- Understanding these new viruses is important for research integrity and diagnostics.