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Quantitation of liver-stage parasites by automated TaqMan real-time PCR

Adam A Witney1, Robert M Anthony, Daniel J Carucci

  • 1Malaria Program, Naval Medical Research Center, Silver Spring, MD, USA.

Methods in Molecular Medicine
|July 20, 2002
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...