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A screening system for enantioselective enzymes based on differential cell growth
Manfred T Reetz1, Carsten J Rüggeberg
1Max-Planck-Institut für Kohlenforschung, D-45470 Mülheim/Ruhr, Germany. reetz@mpi-muelheim.mpg.de
Chemical Communications (Cambridge, England)
|July 20, 2002
Abstract:
The esterase-catalyzed enantioselective hydrolysis of the fluoroacetate of pantolactone leads to fluoroacetic acid, a toxic compound which inhibits the growth of esterase-producing yeast; this forms the basis of an ee-assay.