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Scale-up of recombinant protein purification by hydrophobic interaction chromatography
1Institut für angewandte Mikrobiologie, Universität für Bodenkultur, Vienna, Austria.
Journal of Chromatography
|November 13, 1992
Abstract:
The scale-up of hydrophobic interaction chromatography is described. Human recombinant superoxide dismutase was used as a model. The scale-up was performed by keeping the height to diameter (H/D) ratio of the column constant. The success of scale-up was evaluated by reversed-phase high-performance liquid chromatography of the eluted material. The wrong H/D ratio causes decreased resolution.