In vitro microbicidal activity of W/O/W multiple emulsion for vaginal administration
Related Experiment Video
Updated: Sep 30, 2026

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
Published on: March 3, 2023
G M Tedajo1, S Bouttier, J L Grossiord
1Laboratoire de Physico-Chimie, Pharmacotechnie et Biopharmacie UMR CNRS 8612, Châtenay-Malabry, France.
Abstract:
The microbicidal activity of a W/O/W multiple emulsion destined for vaginal application, containing lactic acid in the internal aqueous phase, octadecylamine (ODA) in the oily phase and benzalkonium chloride (CBZ) in the external aqueous phase was evaluated against three microbial strains: Escherichia coli, Staphylococcus aureus and Candida albicans. The results were different depending on the procedure used. Interpretable results were obtained if only a gentle agitation was used just after the introduction of the microbial suspension to the product. This suggested that vigorous agitation lead to a variable fraction of CBZ or ODA entrapped in the micelles of ethylene and propylene oxide copolymer (COE).
Related Concept Videos
Surface Membrane Barriers
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
Antimicrobial Effectiveness
Hand hygiene
Hand washing...
Chemical Agents for Microbial Control
Microbiota of the Urogenital Tract
Biological Methods for Microbial Control

