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Related Experiment Videos

Hydration, slaving and protein function.

Hans Frauenfelder1, P W Fenimore, B H McMahon

  • 1Center for Non-linear Studies, MS B258, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.

Biophysical Chemistry
|July 20, 2002
PubMed
Summary

Environmental factors like hydration and solvent viscosity significantly impact protein dynamics and function. Experiments show these effects are crucial for understanding globular proteins beyond physiological temperatures.

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Area of Science:

  • Biophysics
  • Structural Biology
  • Biochemistry

Background:

  • Protein dynamics are essential for protein function.
  • Proteins operate within complex biological networks and regulated environments.
  • Environmental factors such as hydration and solvent viscosity critically influence protein behavior.

Purpose of the Study:

  • To elucidate the distinct effects of hydration and solvent viscosity on protein dynamics.
  • To understand how environmental factors modulate protein relaxation and function.
  • To determine if findings from myoglobin are generalizable to other globular proteins.

Main Methods:

  • Utilized wild-type horse and sperm whale myoglobin as model systems.
  • Employed carbon monoxide binding as a probe.

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  • Conducted experiments across a broad range of temperatures to separate hydration and viscosity effects.

    • Main Results:

    • Demonstrated that hydration and solvent viscosity have distinct impacts on protein dynamics.
    • Showed that physiological temperature ranges are insufficient to fully characterize these environmental effects.
    • Observed significant alterations in protein relaxation and function due to hydration and viscosity changes.

    Conclusions:

    • Environmental factors, specifically hydration and solvent viscosity, play a critical role in regulating protein dynamics and function.
    • A comprehensive understanding requires studying proteins across a wide temperature spectrum.
    • The findings are likely applicable to a broad range of globular proteins, not just myoglobin.