Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hydration, slaving and protein function.

Hans Frauenfelder1, P W Fenimore, B H McMahon

  • 1Center for Non-linear Studies, MS B258, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.

Biophysical Chemistry
|July 20, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reply to Wuttke: Our reinterpretation of QENS does not violate scattering theory.

Proceedings of the National Academy of Sciences of the United States of America·2017
Same author

The role of momentum transfer during incoherent neutron scattering is explained by the energy landscape model.

Proceedings of the National Academy of Sciences of the United States of America·2017
Same author

Physical concepts in biology-past and future.

Physical biology·2017
Same author

Coupling Vector-host Dynamics with Weather Geography and Mitigation Measures to Model Rift Valley Fever in Africa.

Mathematical modelling of natural phenomena·2015
Same author

Development of forensic assay signatures for ebolaviruses.

Journal of forensic sciences·2015
Same author

Ask not what physics can do for biology--ask what biology can do for physics.

Physical biology·2014

Environmental factors like hydration and solvent viscosity significantly impact protein dynamics and function. Experiments show these effects are crucial for understanding globular proteins beyond physiological temperatures.

Area of Science:

  • Biophysics
  • Structural Biology
  • Biochemistry

Background:

  • Protein dynamics are essential for protein function.
  • Proteins operate within complex biological networks and regulated environments.
  • Environmental factors such as hydration and solvent viscosity critically influence protein behavior.

Purpose of the Study:

  • To elucidate the distinct effects of hydration and solvent viscosity on protein dynamics.
  • To understand how environmental factors modulate protein relaxation and function.
  • To determine if findings from myoglobin are generalizable to other globular proteins.

Main Methods:

  • Utilized wild-type horse and sperm whale myoglobin as model systems.
  • Employed carbon monoxide binding as a probe.

Related Experiment Videos

  • Conducted experiments across a broad range of temperatures to separate hydration and viscosity effects.

    • Main Results:

    • Demonstrated that hydration and solvent viscosity have distinct impacts on protein dynamics.
    • Showed that physiological temperature ranges are insufficient to fully characterize these environmental effects.
    • Observed significant alterations in protein relaxation and function due to hydration and viscosity changes.

    Conclusions:

    • Environmental factors, specifically hydration and solvent viscosity, play a critical role in regulating protein dynamics and function.
    • A comprehensive understanding requires studying proteins across a wide temperature spectrum.
    • The findings are likely applicable to a broad range of globular proteins, not just myoglobin.