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[Apoptosis of bovine trabecular meshwork cells induced by dexamethasone]
Yajuan Gu1, Shujun Zeng, Pengxin Qiu
1Department of Pharmacology, Sun Yat-sen University, Guangzhou 510089, China. guyajuan@163.net
Objective:
To investigate apoptosis of bovine trabecular meshwork cells induced by dexamethasone.
Methods:
In the incubation method of system in vitro, dexamethasone (1 - 500 mg/L) was added into the syncretic culture solution of the third to fifth generation of bovine trabecular meshwork cells. After 1 - 14 days, the culture solution was observed by phase-contrast microscopy, fluorescence microscopy and transmission electron microscopy, and was studied by DNA laddering and flow cytometric analysis.
Results:
Dexamethasone (125 - 500 mg/L) induced apoptosis of trabecular meshwork cells in a dose-time-dependent manner.
Conclusions:
Apoptosis of cultured bovine trabecular meshwork cells can be induced by dexamethasone, which may be one of the pathogenic mechanisms of steroid-induced glaucoma.
