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[GATA-3 in the development of anterior chamber associated immune deviation]
Tao Fu1, Peizeng Yang, Xiangkun Huang
1Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Objective:
To determine the role of GATA-3 (a transcript factor that has influences on Th2 cell differentiation) in the development of anterior chamber associated immune deviation (ACAID).
Method:
Immunohistochemistry and Western blot were used to determine the localization and relative protein levels of GATA-3 respectively at different time points after anterior chamber injection of 50 microgram interphotoreceptor retinoid binding protein (IRBP).
Result:
GATA-3 was detected weakly in normal spleen and increased significantly at 5, 7, 14 and 21 days after anterior chamber inoculation. It was detected mainly in CD(4)(+) T cells in spleen.
Conclusion:
The expression of GATA-3 increases earlier than development of ACAID. It suggests that GATA-3 play an important role in Th2 commitment during ACAID development.