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[An experimental study on effect of perfluorohexyloctane on corneal endothelial cells]
Xiaoyan Ding1, Chunfang Li, Lin Lu
1Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China. xyding@163.net)
Objective:
To investigate the effect of perfluorohexyloctane on corneal endothelial cells in rabbit eyes.
Methods:
Fifteen New Zealand albino rabbits were divided into two groups: experiment group (F(6)H(8)) and control group (BSS). All rabbits underwent anterior chamber injection of 0.15 ml F(6)H(8) or BSS. Slit-lamp biomicroscopy and corneal endothelium photography were performed pre- and post-injection. Histopathological examination and transmission electron microscopy (TEM) were performed after the rabbits were sacrificed.
Results:
All the corneas were clear. Since 4 weeks postoperatively, the endothelial cells were markedly irregular in size and shape, and the number of endothelial cells was markedly decreased. Multilayered retrocorneal membranes grew gradually from 4 weeks after surgery. Vacuolar degeneration was seen in some endothelial cells. Nuclear degeneration and edema of endoplasmic reticulum were seen in TEM.
Conclusion:
Corneal endothelial cells degenerated after contacting with F(6)H(8) for 4 weeks. As a silicone solvent, F(6)H(8) should be removed out completely after injection. It is not recommended to apply this agent as a new intraocular temponade.