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Antioxidant constituents from Setaria viridis.
Yong Soo Kwon1, Eun Young Kim, Won Jun Kim
1College of Pharmacy, Kangwon National University, Chuncheon, Korea.
Archives of Pharmacal Research
|July 24, 2002
Summary
Extracts from Setaria vindis aerial parts demonstrated significant free radical scavenging. Compounds 4 and 5, identified as orientin 2''-O-xyloside and tricin-7-O-beta-D-glucoside, showed potent antioxidant and anti-lipid peroxidation effects.
Area of Science:
- Phytochemistry
- Pharmacology
- Biochemistry
Background:
- The aerial parts of Setaria vindis are a potential source of bioactive compounds.
- Free radical scavenging and lipid peroxidation are key indicators of oxidative stress and cellular damage.
Purpose of the Study:
- To isolate and identify compounds from Setaria vindis with antioxidant properties.
- To evaluate the free radical scavenging and anti-lipid peroxidation activities of isolated compounds.
Main Methods:
- Extraction and isolation of compounds using solvent fractionation (EtOAc, n-BuOH).
- Identification of compounds via spectral data analysis.
- Assay of free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH).
- Evaluation of inhibition of lipid peroxidation in rat liver microsomes.
Main Results:
- Six compounds were isolated and identified: tricin, p-hydroxycinnamic acid, vitexin 2''-O-xyloside, orientin 2''-O-xyloside (4), tricin-7-O-beta-D-glucoside (5), and vitexin 2"-O-glucoside.
- Compounds 4 and 5 exhibited strong free radical scavenging activity against DPPH.
- Compounds 4 and 5 significantly inhibited non-enzymatic lipid peroxidation in rat liver microsomes.
Conclusions:
- Orientin 2''-O-xyloside (4) and tricin-7-O-beta-D-glucoside (5) possess significant antioxidant and anti-lipid peroxidation properties.
- These findings highlight the potential therapeutic value of Setaria vindis extracts and its isolated compounds in combating oxidative stress.