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Updated: Aug 9, 2026

Measuring Single-Cell Mitochondrial DNA Copy Number and Heteroplasmy Using Digital Droplet Polymerase Chain Reaction
Published on: July 12, 2022
Polymorphism in mitochondrial DNA (mtDNA) of yak (Bos grunniens)
Zheng-Chao Tu1, Huai Qiu, Ya-Ping Zhang
1Kunming Institute of Zoology, The Chinese Academy of Sciences, People's Republic of China.
Abstract:
Mitochondrial DNAs (mtDNA) from 21 yaks (Bos grunniens) were assayed for restriction fragment length polymorphisms by using 20 restriction endonucleases, six of which (AvaI, AvaII, BgIII, EcoRI, HindIII, and HpaI) detected polymorphism. Four different mtDNA haplotypes were identified. Combining this with previous reports about the mtDNA RFLPs of B. indicus and B. taurus, there are obvious differences in mtDNA polymorphism between the yak and other Bos species. We estimated that the divergence times between the ancestor of B. grunniens and the ancestor of B. taurus or B. indicus were about 1.2-2.2 and 1.01-2.02 million years ago, respectively.
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