Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A method for plasmid purification directly from yeast.

Madhu V Singh1, P Anthony Weil

  • 1Department of Molecular Physiology and Biophysics, School of Medicine, Vanderbilt University, Nashville, TN 37232-0615, USA.

Analytical Biochemistry
|July 26, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

miRNA-6236 Regulation of Postischemic Skeletal Muscle Angiogenesis.

Journal of the American Heart Association·2024
Same author

Novel components in the nuclear factor-kappa B (NF-κB) signaling pathways of endothelial cells under hyperglycemic-ischemic conditions.

Frontiers in cardiovascular medicine·2024
Same author

Endothelial Progenitor Cells and Macrophage Subsets Recruitment in Postischemic Mouse Hind Limbs.

Journal of vascular research·2023
Same author

Diabetes mellitus in peripheral artery disease: Beyond a risk factor.

Frontiers in cardiovascular medicine·2023
Same author

BAG3 Attenuates Ischemia-Induced Skeletal Muscle Necroptosis in Diabetic Experimental Peripheral Artery Disease.

International journal of molecular sciences·2022
Same author

Taf2 mediates DNA binding of Taf14.

Nature communications·2022
Same journal

Lysozyme assay using a rationally designed GN4G2 substrate with coupled β-glucosidase reaction.

Analytical biochemistry·2026
Same journal

The long run: A tribute to Arthur Joseph Lawrence Cooper.

Analytical biochemistry·2026
Same journal

Evaluation of a method for affinity measurement using solution equilibrium titration with magnetic beads.

Analytical biochemistry·2026
Same journal

Metabolomics approach using UHPLC/QE-MS for the mechanism of He Xue Ming Mu tablets on non-proliferative diabetic retinopathy.

Analytical biochemistry·2026
Same journal

UniRES-GO: Unified residue-level early fusion of sequence and predicted structure for protein function prediction.

Analytical biochemistry·2026
Same journal

IgG detection by enzyme-linked mass spectrometric assay versus color, fluorescent, ECL in buffer and serum.

Analytical biochemistry·2026
See all related articles

This study presents a rapid, eco-friendly method for purifying yeast plasmids. The technique yields high-quality DNA suitable for various molecular biology applications, including yeast transformation and sequencing.

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Biotechnology

Background:

  • Plasmid purification from Saccharomyces cerevisiae is crucial for molecular biology.
  • Existing methods can be time-consuming, require large volumes, or use toxic chemicals.

Purpose of the Study:

  • To develop a rapid and efficient method for purifying plasmids from yeast.
  • To obtain high-quality plasmid DNA suitable for downstream applications.

Main Methods:

  • Utilizes small culture volumes and commercial bacterial plasmid miniprep kits.
  • Optimized for simultaneous processing of multiple samples.
  • Avoids the use of toxic organic chemicals.

Main Results:

  • Achieves high-quality yeast plasmid DNA purification.

Related Experiment Videos

  • The DNA is suitable for bacterial transformation, yeast transformation, and direct DNA sequencing.
  • Successfully isolates both single-copy CEN/ARS and high-copy 2micron shuttle plasmids.

    • Conclusions:

    • The described technique offers a fast, safe, and versatile approach for plasmid purification from yeast.
    • It is highly applicable to yeast two-hybrid, genetics, and molecular biology experiments.