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Updated: Sep 30, 2026
Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye
Published on: June 20, 2015
[Effect of pressure on proliferation and apoptosis of bovine trabecular meshwork]
1Department of Ophthalmology, Tongji Hospital of Tongji Medical College, Huazhong University of Sciense and Technology, Wuhan 430030, China. xuewei@public.wuhan.cngb.com
Objective:
To investigate the effect of pressure on the expression of cell cycle and apoptosis related gene bcl-2 and bax mRNA by bovine trabecular meshwork cells.
Method:
Twenty mm Hg, 40 mm Hg, 60 mm Hg and 80 mm Hg(1 mm Hg = 0.133 kPa) pressure were given to the cultured trabecular meshwork cells respectively in the treatment groups. No pressure was given in the control groups. Each pressure sustained for 24 and 48 hours. Proliferation index, apoptosis index, bcl-2 and bax mRNA expression of trabecular meshwork cells were detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling, flow cytometry and in situ hybridization.
Results:
In 24 hour groups, there were no significant differences between the apoptosis index of control group and pressure /= 60 mm Hg. In 48 hour groups, apoptosis index, proliferative index and bax mRNA expression had significant differences from that of the control groups when pressure was >/= 40 mm Hg.
Conclusion:
Pressure >/= 40 mm Hg sustaining for a period of time can suppress trabecular meshwork cell proliferation and induce apoptosis through influencing bcl-2 family members.
