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The effect of fibrillar A Beta 1-40 on membrane fluidity and permeability
Ma Xiaocui1, Yinlin Sha, Kechun Lin
1Department of Biophysics, School of Basic Medical Sciences, Peking University, Beijing 100083, PR China.
Abstract:
The time course of A beta fibril formation was characterized using a variety of assays. The effect of A beta on the membrane fluidity and permeability was assessed by monitoring the anisotropy of the fluorescent probe DPH and TMA-DPH and measuring the release of vesicle-entrapped with three different molecular weight fluorescence probes (ANTS/DPX, Calcein, FD-4). The results show fibrils had formed after 4 days. Aggregated A beta may decrease the membrane fluidity and induce the leakage of ANTS and Calcein in a dose dependent manner. These effects may have some relation with A beta neurotoxicity.