Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Progress in Targeted Gene Integration.

Zhu-Hong Li1, De-Pei Liu, Chih-Chuan Liang

  • 1National laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China. liudp@public.east.cn.net

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|July 27, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ACAD8 deficiency promotes pathological cardiac hypertrophy in response to pressure overload by regulating histone isobutyrylation.

Nature communications·2026
Same author

Metabolomics Identified Caloric Restriction-associated Glycerophospholipid Alterations in ApoE-/- Mice.

Genomics, proteomics & bioinformatics·2026
Same author

Redox rhythms promote fitness by modulating ageing-dependent reprogramming.

Nature metabolism·2026
Same author

Correction to: Targeting PDE4B (Phosphodiesterase-4 Subtype B) for Cardioprotection in Acute Myocardial Infarction via Neutrophils and Microcirculation.

Circulation research·2026
Same author

Calcium transfer from the ER to other organelles for optimal signaling in <i>Toxoplasma gondii</i>.

eLife·2025
Same author

Enhanced B cell electroporation efficiency via inhibition of DNA-induced apoptosis and pyroptosis with pan-caspase inhibitor.

Molecular therapy. Methods & clinical development·2025
Same journal

[Prokaryotic expression and characterization of rice Rac protein osRACB].

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica·2003
Same journal

[Purification of a big defensin from Ruditapes philippinesis and its antibacterial activity].

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica·2003
Same journal

[Expression, purification and identification of recombinant SARS coronavirus membrane protein].

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica·2003
Same journal

[Molecular cloning of mTSARG3 gene related to apoptosis in mouse spermatogenic cells].

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica·2003
Same journal

[The immunopotentiation of human B lymphocyte stimulator C-terminal peptide].

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica·2003
Same journal

[Cloning, eukaryotic expression and function assay of recombinant leukemia inhibitory factor gene LIF].

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica·2003
See all related articles

Gene therapy aims to correct genetic disorders by introducing genetic material into cells. Homologous recombination offers a promising strategy for permanent, controlled gene expression, advancing genetic disorder correction.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Current gene therapy vectors often fail to achieve permanent and appropriately regulated gene expression.
  • Achieving stable, controlled gene expression is crucial for effective genetic disorder correction.

Purpose of the Study:

  • To highlight homologous recombination as a superior strategy for gene therapy.
  • To discuss the potential of targeted gene integration for correcting genetic disorders.

Main Methods:

  • Focuses on the principles of homologous recombination for targeted DNA integration.
  • Reviews recent advancements enabling precise gene insertion into the genome.

Main Results:

  • Homologous recombination allows for gene expression controlled by natural cellular elements, ensuring appropriate levels.

Related Experiment Videos

  • Targeted integration via homologous recombination facilitates the correction of genetic disorders.

    • Conclusions:

    • Homologous recombination represents the optimal strategy for human gene therapy due to its precise and regulated gene delivery.
    • Recent progress in homologous recombination techniques paves the way for effective correction of genetic disorders through targeted gene integration.