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Cloning, Sequencing and Expression of Human Copper, Zinc-superoxide Dismutase cDNA
Hui-Juan Shi1, Li-Qiang Fan, Dong-Zhi Wei
1Research Institute of Biochemistry, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. qsyuan@ecust.edu.cn
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|July 27, 2002
Abstract:
The cDNA encoding the human copper, zinc-superoxide dismutase(Cu, Zn-SOD) was amplified from the human liver by RT-PCR and sequenced. The cloned human Cu, Zn-SOD cDNA was ligated into expression vector pET-22b(+) under T7 promotor. After 3 h induction with 1 mmol/L IPTG, human Cu, Zn-SOD was highly expressed in E.coli BL21(DE3). The expression product was up to 30% of the total protein of the bacteria in soluble form, which had specific SOD activity.