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Published on: October 4, 2024
Transformation of Neomycin Resistance Gene (neo(R)) into Silkworm (Bombyx mori.L.)
Xiu Chen1, Yun Zhao, Feng Zhang
1Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. lucd@sunm.shcnc.ac.cn
Abstract:
Neomycin resistance gene (neo(R)) flanked by 5' and 3' regions of fibroin H-chain gene of silkworm (Bombyx mori.L.) was transferred into eggs of silkworm by gene gun in the early period of fertilization. The larvae were fed with an artificial diet containing neomycin in early 24 hours post transfettion, and some of them survived. The neo(R) encoding sequence in G(2) generation derived from the survivals was detected by Southern blotting. The results indicated that neo(R) could be used as a selective marker for studies on transgenic silkworm.
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