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Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies
Published on: March 19, 2014
Production of recombinant human calcitonin from silkworm (B. mori) larvae infected by baculovirus
Guan-Zhen Yang1, Zhen-zhen Chen, Da-Fu Cui
1Institute of Biochemistry and Cell Biology, Shanghai Institutes of Biological Science, Shanghai, 200031, China.
Abstract:
A synthetic modified gene encoding the human calcitonin analog (hmCT) was expressed by use of the baculovirus expression system. After injection with recombinant baculoviruses, the hmCT-GST fusion protein was produced within the silkworm larvae. The fusion protein was purified by affinity chromatography. Biological activity of hmCT for hypercalcemic effect was determined in normal rats.
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