Methanol-induced biological nutrient removal kinetics in a full-scale sequencing batch reactor

The primary goal of this research was to determine the potential for denitrification and phosphorus removal of a full-scale sequencing batch reactor (SBR), with and without the use of methanol as an external carbon source. The control SBR, without methanol addition, achieved negligible denitrification. Two denitrification rates were observed in the experimental SBR, with methanol addition, an initial fast rate and a slower second rate. The denitrification rate during the first rate period increased with increasing methanol concentration, until a maximum denitrification rate of approximately 19 mg NOx-N/g MLVSS/day was attained. Following the depletion of the methanol, denitrification reactions probably continued by using the available natural carbon in the influent, resulting in a slower, second denitrification rate. Biological phosphorus uptake and release was significant only in the SBR with methanol addition. Methanol was probably not utilized as the carbon source for the enhanced biological phosphorus removal (EBPR) process. However, methanol addition was critical, since it depleted the available nitrates and thus allowed EBPR to take place.