Related Experiment Videos
Bacillus funiculus sp. nov., novel filamentous isolates from activated sludge
Vasudevan P Ajithkumar1, Bindu Ajithkumar, Ryozo Iriye
1Laboratory of Ecological and Toxicological Chemistry, Faculty of Agriculture, Shinshu University, Kamiina, Nagano, Japan.
Summary
A new Bacillus species, Bacillus funiculus sp. nov., is proposed based on DNA-DNA relatedness and chemotaxonomic data. This filamentous bacterium exhibits unique budding growth from spore-like resting cells.
Area of Science:
- Microbiology
- Bacterial Taxonomy
- Molecular Biology
Background:
- A novel filamentous Bacillus strain, NAF001T, was previously identified.
- This strain produces endospores and unique spore-like resting cells that outgrow by budding.
- Phylogenetic analysis suggested it represents a new species within the Bacillus genus.
Purpose of the Study:
- To authenticate the creation of a novel Bacillus species.
- To determine the DNA-DNA relatedness of strain NAF001T to other Bacillus species.
- To characterize the whole-cell fatty acid and quinone profiles of strain NAF001T.
Main Methods:
- DNA-DNA hybridization experiments were conducted.
- Whole-cell fatty acid composition was analyzed.
- Quinone profiles were determined.
- Electron microscopy was used to visualize filament binding.
Main Results:
- Strain NAF001T showed low DNA-DNA relatedness to recognized Bacillus species, supporting its status as a new species.
- Fatty acid and quinone profiles were consistent with its placement within the Bacillus genus.
- Electron microscopy revealed specific binding points enabling the formation of extremely long filaments.
Conclusions:
- The data authenticate the proposal of Bacillus funiculus sp. nov. as a novel species.
- Bacillus funiculus sp. nov. is characterized by its filamentous growth and unique budding from resting cells.
- The study provides a comprehensive taxonomic description of this new bacterial species.