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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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Related Experiment Video

Updated: Jul 3, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Protein splicing triggered by a small molecule.

Henning D Mootz1, Tom W Muir

  • 1Laboratory of Synthetic Protein Chemistry, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.

Journal of the American Chemical Society
|August 1, 2002
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel method to control protein function using small molecules. This technique harnesses protein splicing, enabling precise temporal regulation of protein activity in response to rapamycin.

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Area of Science:

  • Chemical Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Controlling protein function with small molecules offers temporal precision difficult to achieve with genetic methods.
  • Current strategies often involve direct binding to protein active sites or inducing structural changes.
  • Altering a protein's primary structure via small molecules presents an alternative control mechanism.

Purpose of the Study:

  • To introduce a new technique for controlling protein function using small molecules.
  • To leverage protein splicing for inducible control of protein activity.
  • To develop a generalizable method for small-molecule-mediated protein regulation.

Main Methods:

  • Harnessing protein splicing, a natural posttranslational process.
  • Designing a system where protein splicing is triggered by the small molecule rapamycin.
  • Developing a method that is independent of the specific N- and C-extein sequences.

Main Results:

  • Demonstrated a technique enabling protein splicing exclusively in the presence of rapamycin.
  • Established a novel mechanism for small-molecule-induced protein functional control.
  • Showcased the potential for broad applicability across different protein targets.

Conclusions:

  • The developed technique provides a generalizable platform for controlling protein function with small molecules.
  • This approach offers precise temporal regulation of protein activity.
  • This method advances the field of chemical genetics by introducing a new tool for protein manipulation.