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Related Experiment Videos

Cocaine-amphetamine regulated transcript (CART) expression is not regulated by amphetamine.

Niels Vrang1, Philip J Larsen, Peter Kristensen

  • 1Rheoscience, Glerupvej 2, 2610 Rodovre, Denmark.

Neuroreport
|August 2, 2002
PubMed
Summary

Amphetamine does not appear to regulate Cocaine-amphetamine-regulated transcript (CART) mRNA levels in the rat brain, contrary to previous findings. This challenges the name of this neuropeptide, which is abundant in the hypothalamus.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Endocrinology

Background:

  • Cocaine-amphetamine-regulated transcript (CART) is highly expressed in the rat hypothalamus.
  • CART peptides are linked to feeding behavior and HPA-axis regulation.
  • Previous studies suggested CART mRNA is induced by amphetamine in the striatum.

Purpose of the Study:

  • To re-examine the effects of amphetamine on CART mRNA expression in the rat forebrain and hypothalamus.
  • To investigate the regulation of CART expression in response to amphetamine treatment.

Main Methods:

  • In situ hybridization was used to analyze CART mRNA levels.
  • Experiments utilized the same amphetamine treatment paradigm as prior studies.

Main Results:

Related Experiment Videos

  • No regulation of CART mRNA levels by amphetamine was observed in either the forebrain or hypothalamus.
  • The findings contradict previous reports on amphetamine's effect on CART expression.

Conclusions:

  • The results question the validity of the acronym 'Cocaine-amphetamine-regulated transcript' for this predominantly hypothalamic neuropeptide.
  • Further research is needed to clarify the regulatory mechanisms and functional roles of CART.