[Effect of trypsin on endogenous RNA synthesis of cell nuclei]

The effect of trypsin on endogenous RNA-polymerase activity was studied in the rat liver and kidney cell nuclei. It is shown that the endogenic ability for RNA synthesis of the liver nuclei is higher than that of the kidney ones. Trypsin effect causing chromatin histone hydrolysis and deblocking of the genome-primer results in the above 4-fold stimulation of RNA synthesis in both types of nuclei. Ammonium sulphate causes an additional growth of nuclei transcription ability. On the basis of the data on determining the endogenic RNA-polymerase activity in the medium with MgCl2, on the one hand, and in the medium with MnCl2 and ammonium sulphate, on the other, a conclusion is drawn that caryoplasmatic form of RNA-polymerase in the kidney nuclei prevails as compared with the liver nuclei.