[A rapid method for preparative separation of lipoproteins into main groups by density gradient ultracentrifugation]

A rapid method was developed for separation of lipoproteins by flotation in an ultracentrifuge using a four-step density gradient 1.006, 1.063, 1.250 and 1.300 g/ml. A satisfactory fractionation of serum lipoproteins was carried out by single ultracentrifugation within 4-5 hrs at 216,000 g.