Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Profiling protein function with small molecule microarrays.

Nicolas Winssinger1, Scott Ficarro, Peter G Schultz

  • 1Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, CA 92121, USA.

Proceedings of the National Academy of Sciences of the United States of America
|August 9, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Caged Monomethyl Auristatin F (MMAF) for Cell-Specific Activation Using a 488-nm-Optimized Photolabile Group.

JACS Au·2026
Same author

Child-Directed Marketing on Product Packages of Sweet Snacks, Cereals, and Yogurts: Content Analysis and Association With Nutrition Quality.

Journal of nutrition education and behavior·2026
Same author

DNA-drug conjugates enable logic-gated drug delivery amplified by hybridization chain reactions.

Nature biotechnology·2026
Same author

One-pot multi-substrate screening of ligation reactions using PNA tags.

Chemical science·2026
Same author

Exposure to Food, Beverage, and Alcohol Advertising on Spanish-Language Television Among Children, Adolescents, and Adults in the US, 2022.

Journal of nutrition education and behavior·2026
Same author

Adolescent Exposure to Food and Alcohol Advertising on TV in the U.S., 2013-2022.

AJPM focus·2025
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

This study introduces a novel small molecule microarray for profiling protease activity in cell lysates. It successfully detects caspase activation during apoptosis and identifies inhibitors, offering new insights into cellular processes.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Protein function regulation is vital for cellular processes.
  • Analyzing protein activity, not just abundance, offers deeper biological insights.
  • Current methods may not fully capture dynamic protein functional changes.

Purpose of the Study:

  • To develop and apply a small molecule microarray for genome-wide activity-based protein profiling.
  • To demonstrate the utility of this technology in studying protease activation and inhibition.
  • To provide a new tool for understanding complex biological processes at the proteome level.

Main Methods:

  • Utilized a spatially addressable small molecule microarray.
  • Performed activity-based profiling on crude cell lysates.

Related Experiment Videos

  • Applied the technology to study protease activation, specifically caspases, during apoptosis.

    • Main Results:

    • Successfully detected caspase activation in response to apoptosis induction.
    • Characterized the specific activated caspase using the microarray.
    • Identified a small molecule inhibitor that reversed the caspase-executed apoptotic phenotype.

    Conclusions:

    • Small molecule microarray profiling is a powerful tool for analyzing protease activity in complex biological samples.
    • This technology enables the identification of functional proteases and their inhibitors.
    • The approach provides valuable insights into cellular signaling pathways like apoptosis.