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Nucleotide Sequence and Characterization of the p10 Gene of Spodoptera litura Nuclear Polyhedrosis Virus
Yong-Jie Wei1, Qi-Xin Long, Shang-Wu Chen
1State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, Guangzhou 510275, China. ls07@zsu.edu.cn
Abstract:
The p10 gene was localized within the 3.0 kbp BamH I fragment of the SINPV genome. With a coding sequence of 318 nucleotides, corresponding to a protein of 105 amino acids, the p10 gene of SINPV is the longest p10 gene identified so far in NPVs. The SINPV p10 gene is also distinct in having two A/TTTGTA motifs in the promoter region. With 10 heptad repeats, the SINPV P10 protein is probably forming a relatively large coiled-coil structure. The organization of SINPV ORF552-p10 gene-ORF945 cluster is collinear with that of SpliNPV and their corresponding amino acid sequences of each ORF share high similarity. This indicates that SINPV and SpliNPV belong to a homologous baculovirus group.
