Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell repopulation in vascularized bone grafts.

Keiichi Muramatsu1, Allen T Bishop

  • 1College of Engineering and Computer Science, University of Tennessee at Chattanooga, 37403, USA.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
|August 10, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Glenohumeral arthrodesis improves elbow flexion in patients with adult brachial plexus injuries.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2026
Same author

Flexor Carpi Radialis Tendon Rupture from Scapho-Trapezio-Trapezoid Osteoarthritis: A Case Report and Review of Literature.

The journal of hand surgery Asian-Pacific volume·2026
Same author

Nonbrachial Plexopathy Diagnoses Seen in a Brachial Plexus Injury Specialty Clinic.

Journal of hand surgery global online·2026
Same author

Late-Onset Reactivation of Hand Macrodactyly in a Septuagenarian.

The journal of hand surgery Asian-Pacific volume·2026
Same author

Vertical Locking of the Thumb Metacarpophalangeal Joint from Metacarpal Osteophyte: A Case Report and Review of Literature.

The journal of hand surgery Asian-Pacific volume·2026
Same author

First Metacarpal Extension Osteotomy by Use of a New Anatomical Shamoji Plate for Base of Thumb Arthritis: Short-Term Results.

The journal of hand surgery Asian-Pacific volume·2026
Same journal

Gait Characteristics and Intersegmental Foot and Ankle Motion in Patients With Neglected Achilles Tendon Rupture.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2026
Same journal

A Biomechanical Proof-of-Concept Study of Scaffold-Augmented Distraction Osteogenesis Using 3D-Printed Hyperelastic Bone™ in a Rabbit Tibial Lengthening Model.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2026
Same journal

Predicting Primary Stability of Stemless TSA From Preoperative Bone Density and Surgical Factors.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2026
Same journal

Locking Compression Plate Versus Dynamic or Limited Contact Dynamic Compression Plate Fixation for Forearm Fractures: A Systematic Review and Meta-Analysis.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2026
Same journal

Synovial Fluid Biomarkers Reflect Pre- and Postoperative Clinical Features in Rotator Cuff Tears, Independent of Tear Size.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2026
Same journal

Assessing Lateral Patellofemoral Dislocations on MRI: A Novel Contusion Grading Approach for Predicting Recurrence and Prognosis.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2026
See all related articles

Vascularized bone grafts are gradually repopulated by recipient cells, with donor cells diminishing over time. Y-chromosome-specific PCR effectively tracks cell lineage and repopulation in these bone grafts.

Area of Science:

  • Regenerative Medicine
  • Orthopedic Surgery
  • Transplantation Biology

Background:

  • Osteocyte viability is crucial following vascularized bone autografts.
  • Cellular repopulation involves graft-derived or host-derived cells.
  • Understanding cell dynamics is key for successful bone grafting.

Purpose of the Study:

  • To define the process of cell repopulation in vascularized bone grafts.
  • To quantify the contribution of host-derived cells over time.
  • To evaluate Y-chromosome-specific PCR for lineage tracing.

Main Methods:

  • Sex-mismatched vascularized tibial isografts in Lewis rats (female to male, male to female).
  • Immunosuppression with FK506 in a subset of recipients.
  • Assessment via radiography, histology, and Y-chromosome/autosome-specific PCR up to 24 weeks.

Related Experiment Videos

Main Results:

  • Host-derived cells were not detected before six weeks post-transplant.
  • The proportion of host-derived cells increased gradually over 24 weeks.
  • By 24 weeks, only 0.1-1.0% of graft-derived cells remained; periosteal hypertrophy linked to donor cells, later remodeling to host cells.

Conclusions:

  • Y-chromosome-specific PCR is a valuable tool for tracking cell lineage in sex-mismatched bone grafts.
  • Vascularized bone grafts undergo progressive repopulation by host-derived cells.
  • Donor cells dominate early graft survival, while host cells drive later remodeling.