Related Experiment Video
Updated: Sep 30, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Effect of Type I Collagen on the Susceptibility of Low Density Lipoprotein to Oxidation
En-Hui Wei1, Qi Chen, Xiu-Ying Chen
1Atherosclerosis Research Center, Nanjing Medical University, Nanjing 210029, China.
Abstract:
In order to observe the effect of type I collagen on the susceptibility of low density lipoprotein (LDL) to oxidation, the collagen gel system was established in vitro. The presence of collagen resulted in a protracted lag phase as well as a decreased production of the thiobarbituric acid reactive substance (TBARS) when LDL was oxidized by the addition of copper ion. The relative electrophoretic mobilities (REM) of LDL in the treated group were far lower than those in the control group; while during the 2,2'-amidinopropane hydrochloride(AAPH)-catalyzed oxidation of LDL, neither the lag phase nor the maximum content of the output of TBARS were significantly affected by the presence of collagen. These results suggest that collagen may decrease the susceptibility of LDL to copper-catalyzed oxidation the mechanism of which may not involve the capture of free radicals.
Related Concept Videos
Lipid-derived Compounds in the Human Body
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin, delayed...
Atherosclerosis I: Introduction
Cholesterol: Significance and Regulation
Considering cholesterol and...
Lipids: Dietary Sources and Requirements
Coronary Artery Disease II: Pathophysiology
Blood Studies for Cardiovascular System III: Serum Lipid Profile
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
