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Self-regulation of rat liver GAP junction by phosphorylation
Paramita Ghosh1, Subhendu Ghosh, Sudipto Das
1Department of Biophysics, University of Delhi South Campus, Benito Juarez Road, New Delhi-110021,India.
Biochimica Et Biophysica Acta
|August 15, 2002
Abstract:
We have studied the functioning of rat liver Connexin 32 (C x 32) at the single channel level in presence of ATP. It was observed that ATP regulates the functioning of the channel by running down the junctional conductance. A non-specific exogenous protein phosphatase (alkaline phosphatase) reversed the rundown of junctional activity to its normal functioning state. Autoradiograhic studies demonstrate autophosphorylation of rat liver C x 32. These findings indicate a self-regulatory mechanism of the channel.