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Related Experiment Videos

Mitotic exit: the Cdc14 double cross.

Marco Geymonat1, Sanne Jensen, Leland H Johnston

  • 1Division of Yeast Genetics, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.

Current Biology : CB
|August 15, 2002
PubMed
Summary
This summary is machine-generated.

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The cell division protein Cdc14 is released in two waves during anaphase, with early and late pulses having opposing effects on the MEN pathway, a key regulator of cell cycle progression.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Cdc14 phosphatase is a key regulator of cell cycle progression.
  • Its release from the nucleolus is essential for exit from mitosis.
  • This release occurs in two distinct waves during anaphase, controlled by the FEAR and MEN pathways.

Purpose of the Study:

  • To investigate the localization of Cdc14 released in early anaphase.
  • To understand the distinct roles of the two Cdc14 release waves in regulating cell division.
  • To elucidate the opposing effects of early and late Cdc14 pulses on the MEN pathway.

Main Methods:

  • Immunofluorescence microscopy to track Cdc14 localization.
  • Analysis of cell division mutants.
  • Biochemical assays to assess MEN pathway activation.

Related Experiment Videos

Main Results:

  • Cdc14 released in early anaphase localizes to the spindle pole body.
  • The two waves of Cdc14 release exert opposing influences on MEN pathway activation.
  • Early anaphase Cdc14 release promotes MEN activation, while late anaphase release inhibits it.

Conclusions:

  • The spatiotemporal release of Cdc14 is crucial for precise cell cycle control.
  • Opposing roles of early and late Cdc14 pulses ensure proper mitotic exit.
  • Cdc14's dual function highlights complex regulatory mechanisms in cell division.