Evaluation of cultivation performance of S. cerevisiae strains expressing GLP-1-GIP peptide precursors
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Published on: December 13, 2014
Ioannis Voulgaris1, Anders Nygaard Nielsen2, Tine Petersen2
1Therapeutic Discovery, Novo Nordisk A/S, Novo Nordisk Park 1, 2760, Måløv, Denmark. IVO@novonordisk.com.
Abstract:
Strains of S. cerevisiae expressing GLP-1-GIP peptide precursors with an N-terminal extension demonstrate up to 29% higher molar productivity during continuous cultivation compared to non-extended counterparts. Additionally, inactivation of the S. cerevisiae AMN1 gene, through deletion, effectively reduces broth viscosity by more than 45%, thereby enhancing oxygen transfer without adversely affecting cell metabolism or product titer. These results highlight the advantages of molecular modifications and genetic alterations for improving the production efficiency of GLP-1 receptor agonists, addressing critical challenges related to recombinant expression, viscosity and oxygen transfer in bioprocessing.
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