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Regulation of MTP expression in developing swine.
Song Lu1, Mark Huffman, Ying Yao
1Children's Foundation Research Center of Memphis, Le Bonheur Children's Medical Center, Memphis, TN 38103, USA.
Journal of Lipid Research
|August 15, 2002
Summary
Microsomal triglyceride transfer protein (MTP) expression in swine is high during fetal development and influenced by diet postnatally. Fatty acids and glucocorticoids play roles in regulating MTP expression.
Area of Science:
- Biochemistry
- Developmental Biology
- Animal Science
Background:
- Microsomal triglyceride transfer protein (MTP) is crucial for lipid metabolism.
- Understanding MTP's developmental regulation is key to comprehending lipid absorption and transport.
Purpose of the Study:
- To investigate the developmental expression of MTP large subunit mRNA and protein in swine.
- To explore the influence of diet and regulatory factors on MTP expression.
Main Methods:
- Collected small intestine and liver samples from swine at various developmental stages (fetal, newborn, suckling, weanling).
- Analyzed MTP mRNA and protein expression using molecular techniques.
- Utilized a swine enterocyte cell line (IPEC-1) to study the effects of dexamethasone and fatty acids.
Main Results:
- MTP mRNA and protein expression were high in fetal intestine and liver, declining postnatally.
- Postnatal jejunal MTP expression correlated with high-fat diet intake and decreased after weaning.
- Dietary triacylglycerol levels affected MTP mRNA in piglets; fatty acids modulated MTP expression in cell culture.
Conclusions:
- MTP expression during swine development is governed by genetic programming and influenced by dietary lipid quantity and composition.
- Glucocorticoids have a limited role in regulating MTP expression in enterocytes.
- Dietary factors significantly impact MTP expression, highlighting their role in lipid homeostasis.