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Related Experiment Video

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Candida albicans Biofilm Development on Medically-relevant Foreign Bodies in a Mouse Subcutaneous Model Followed by Bioluminescence Imaging
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[Quantitative cultures from catheters, using the Brun Buisson technique].

R Soloaga1, L Guelfand, S Manganello

  • 1División Laboratorio, Sección Microbiología, Departamento de Investigación Clínica IUCB, Fundación Favaloro, Buenos Aires, Argentina. rsoloaga@drwebsa.com.ar

Revista Argentina De Microbiologia
|August 16, 2002
PubMed
Summary

Optimal incubation for quantitative catheter cultures involves aerobic or 5-10% CO2 atmospheres, with no benefit from incubation beyond 48 hours. This finding aids in accurate catheter-related bloodstream infection diagnosis.

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Area of Science:

  • Medical Microbiology
  • Infectious Diseases
  • Clinical Diagnostics

Background:

  • Catheter-related bloodstream infections (CRBSIs) are a significant concern in healthcare settings.
  • Accurate quantitative culture methods are crucial for diagnosing CRBSIs.
  • The Brun Buisson method using vortex agitation is a standard technique for catheter processing.

Purpose of the Study:

  • To determine optimal incubation conditions (time and atmosphere) for quantitative catheter cultures.
  • To evaluate the impact of different incubation atmospheres on microbial recovery.
  • To establish the reliability of colony counts at various incubation durations.

Main Methods:

  • Quantitative cultures of 138 positive catheters processed using the Brun Buisson vortex agitation technique.
  • Comparison of colony counts from aerobic versus 5-10% CO2 incubation atmospheres.
  • Evaluation of colony counts at 48 hours, 3 days, and 7 days of incubation.

Main Results:

  • A very good correlation was observed between aerobic and 5-10% CO2 atmospheres (p = 0.27; r2 = 0.9268).
  • Incubating plates beyond 48 hours showed no additional advantage.
  • Excellent correlation was found between colony counts at 2 vs. 3 days (p = 0.10; r2 = 0.9996) and 2 days vs. 7 days (p = 0.31; r2 = 0.9995).

Conclusions:

  • Optimal incubation for quantitative catheter cultures can be achieved using either aerobic or 5-10% CO2 atmospheres.
  • A 48-hour incubation period is sufficient for accurate quantitative cultures of catheters.
  • These findings support efficient and reliable diagnostic protocols for catheter-related infections.