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The effect of interleukin-6 on L-selectin levels on polymorphonuclear leukocytes

Tatsushi Suwa1, James C Hogg, Kevin B Quinlan

  • 1McDonald Research Laboratory and iCAPTURE Centre, University of British Columbia, St. Paul's Hospital, Vancouver, British Columbia, Canada V6Z 1Y6.

American Journal of Physiology. Heart and Circulatory Physiology
|August 16, 2002
PubMed

Insights

Interleukin-6 (IL-6) reduces L-selectin levels on circulating polymorphonuclear leukocytes (PMN). This immune response involves demargination and accelerated release of PMN with lower L-selectin from the marrow.

Area of Science:

  • Immunology
  • Hematology

Background:

  • Interleukin-6 (IL-6) influences polymorphonuclear leukocyte (PMN) kinetics and release from bone marrow.
  • IL-6's effect on PMN L-selectin levels differs from other inflammatory stimuli.

Purpose of the Study:

  • To investigate the impact of IL-6 on L-selectin expression in rabbit PMN.
  • To elucidate the mechanisms by which IL-6 affects PMN L-selectin levels.

Main Methods:

  • Administration of recombinant human IL-6 to rabbits.
  • Quantification of L-selectin levels on circulating and marrow PMN over time.

Main Results:

  • IL-6 treatment significantly decreased L-selectin levels on circulating PMN from 3 to 12 hours post-treatment.
  • L-selectin levels were reduced on PMN in circulation (up to 4 hours) and newly released from marrow (up to 12 hours).
  • PMN within the bone marrow also showed decreased L-selectin levels for up to 6 hours after IL-6 administration.

Conclusions:

  • IL-6 downregulates L-selectin on circulating PMN through demargination and enhanced marrow release.
  • The sustained reduction in PMN L-selectin by IL-6 may impact leukocyte recruitment to inflammatory sites.

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