Related Experiment Video
Updated: Aug 10, 2026

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
Published on: November 1, 2011
Size and sequence variability of the Arabis mosaic virus protein 2A
Thierry Wetzel1, M Fuchs, M Bobko
1Centrum Gruene Gentechnik, Staatliche Lehr- und Forschungsanstalt, Breitenweg 71, D-67435 Neustadt an der Weinstrasse, Germany. twetzel.slfa-nw@agrarinfo.rlp.de
Abstract:
The RNA 2 of the nepovirus Arabis mosaic virus (ArMV) encodes a polyprotein from which protein 2A is released by proteolytic cleavage at the N-terminus. The 2A gene of 19 ArMV isolates from different geographical origin and 9 distinct natural hosts was amplified by RT/PCR and subsequently cloned and sequenced. These 19 isolates and those from databanks were classified into four groups based on the size of the protein 2A which ranged from 233 to 280 amino acids, and sequence identities. Sequence variability was mainly located in the N-terminus of the proteins, whereas the core region and the C-terminus were conserved.
Related Concept Videos
Viral Recombination
Leaky Scanning
Size and Structure of Viral Genomes
Viruses of Archaea
The Antiviral System of Bacteria and Archaea: CRISPR
Inhibitors of Virion Maturation and Assembly

