Related Experiment Videos
Effects of granulocyte colony-stimulating factor (g-csf) on neutrophil phagocytosis during experimental obstructive
Bekir Aldemir1, Zafer Canturk, Gul Ilbay
1Kocaeli University, Medical School, Department of Genaral Surgery, Kocaeli, Turkey.
Background:
Obstruction ofthe extrahepati(biliary tree produces profound depression of many components ofthe immune system. G-CSF improves diseasedfiinction fneutrophils in various conditions. In this study, we planned to investigate the changes on neutrophil phagocytosis in obstruct;ve jaundice and the effect ofG-CSF adm;nistration on thisfiinction.
Methods:
Rats were divided into 5 groups as follows: the sham group and four other groups that underwent double ligation and division of common bile duct. Two of these four groups (Group 3 and 5) received G-CSF during experiment. Neutrophil hagocytosis index was determinedforgroup2and 3 attheend ofthe 15 daysand forgroup 1, 4and 5 attheend of the21 days.
Results:
Neutrophil phagocytosis index significantly increased at the end ofthe 15th day after the bile duct ligation (Group 2) and significantly decreased at the end ofthe 21th day after the bile duct ligation (Group 4). Neutrophil phagocytosis index in G-CSF-treated groups was significantly increased at the end ofthe 15'h days (Group 3) and increased at the end of the 21th day (Group 5).
Conclution:
As a result, neutrophil phagocytosis index is improved if G-CSF is administered later in the course of prolonged jaundice.