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Updated: Aug 25, 2026
Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination
Published on: July 4, 2007
Genetic characterization of dengue virus type 3 isolates in the State of Rio de Janeiro, 2001
M P Miagostovich1, F B dos Santos, T S de Simone
1Departamento de Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil. marizepm@ioc.fiocruz.br
Abstract:
The genetic characterization of dengue virus type 3 (DEN-3) strains isolated from autochthonous cases in the State of Rio de Janeiro, Brazil, in 2001 is presented. Restriction site-specific (RSS)-PCR performed on 22 strains classified the Brazilian DEN-3 viruses as subtype C, a subtype that contains viruses from Sri Lanka, India, Africa and recent isolates from Central America. Nucleic acid sequencing (positions 278 to 2550) of one DEN-3 strain confirmed the origin of these strains, since genotype III - classified by sequencing - and RSS-PCR subtype C are correlated. This genetic subtype has been associated with hemorrhagic dengue epidemics and the information provided here could be useful to implement appropriate prevention and control measures.
