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Related Experiment Videos

On-chip absorption measurements using an integrated waveguide.

Bryan G Splawn1, Fred E Lytle

  • 1Chemistry Department, 1393 Brown Laboratories, Purdue University, West Lafayette, IN 47907-1393, USA.

Analytical and Bioanalytical Chemistry
|August 20, 2002
PubMed
Summary

This study integrates absorption measurement with chip-based electrophoresis using novel square hollow waveguides. These waveguides demonstrate efficient light guiding and enable sensitive detection of fluorescent dyes.

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Area of Science:

  • Biophotonics
  • Analytical Chemistry
  • Microfluidics

Background:

  • Chip-based electrophoresis offers miniaturized separation capabilities.
  • Integrating optical absorption measurements enhances analytical sensitivity in microfluidic devices.
  • Traditional waveguides face challenges in microfluidic integration due to refractive index mismatches.

Purpose of the Study:

  • To develop and evaluate square hollow waveguides for integrated absorption measurements in chip-based electrophoresis.
  • To demonstrate the capability of these waveguides for guiding light and detecting fluorescent analytes.
  • To assess the detection limits achievable with this integrated system.

Main Methods:

  • Fabrication of square hollow waveguides (50x50 microm) in poly-dimethylsiloxane (PDMS) replicated from a silicon master.

Related Experiment Videos

  • Utilizing light at 488 nm guided by reflection at the air-PDMS interface, bypassing total internal reflection.
  • Demonstrating separation of fluorescein and BODIPY dyes using chip-based electrophoresis coupled with optical detection.

    • Main Results:

    • Achieved 60% waveguide efficiency over a 3.2 cm distance.
    • Successfully separated fluorescein and BODIPY.
    • Obtained a detection limit of 200 microM fluorescein (S/N=3) with a 50 microm pathlength and photocell detector.

    Conclusions:

    • Square hollow waveguides are effective for integrating absorption measurements with chip-based electrophoresis.
    • The air-PDMS interface provides efficient light guiding in these waveguides.
    • This integrated system shows promise for sensitive detection in microfluidic analytical devices.