Activation of lipase by a factor present in the gall-bladder epithelium

The action of a bovine gall-bladder extract is studied on two lipolytic systems: the pancreas lipase and the plasma lipoproteinlipase. For pancreas lipase the lipolytic activity of pancreas homogenates of different species is evaluated in the presence of different quantities of gall-bladder extract. For plasma lipoproteinlipase, the enzyme is induced by injection of optimal doses of heparin associated with different quantities of gall-bladder extract and the activity is evaluated by measuring the clarifying power of the plasmas of the treated animals. The results confirm that the gall-bladder contains a factor activating the pancreas lipase and put in evidence some differences between lipase activities in different species. For plasma lipolytic activation, the action of the gall-bladder extract is particularly evident in the inductive phase of lipoproteinlipase, according to a typical process of saturation.