Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interactions between integrase and excisionase in the phage lambda excisive nucleoprotein complex.

Eun Hee Cho1, Richard I Gumport, Jeffrey F Gardner

  • 1Department of Science Education, Chosun University, Kwangju, Korea.

Journal of Bacteriology
|August 24, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transition metal-catalyzed regioselective functionalization of carbazoles and indolines with maleimides.

Organic & biomolecular chemistry·2022
Same author

Questionnaire-based Survey of Demographic and Clinical Characteristics, Health Behaviors, and Mental Health of Young Korean Adults with Early-onset Diabetes.

Journal of Korean medical science·2021
Same author

Comparison of the predictive validity of three fall risk assessment tools and analysis of fall-risk factors at a tertiary teaching hospital.

Journal of clinical nursing·2020
Same author

Histone Deacetylase 9: Its Role in the Pathogenesis of Diabetes and Other Chronic Diseases.

Diabetes & metabolism journal·2020
Same author

Soluble Dipeptidyl Peptidase-4 Levels Are Associated with Decreased Renal Function in Patients with Type 2 Diabetes Mellitus.

Diabetes & metabolism journal·2018
Same author

Update on the Aldosterone Resolution Score and Lateralization in Patients with Primary Aldosteronism.

Endocrinology and metabolism (Seoul, Korea)·2018

Bacteriophage lambda integrase (Int) and excisionase (Xis) interact. This study reveals that the amino-terminal domain of Int binds Xis, clarifying their roles in phage DNA recombination.

Area of Science:

  • Molecular Biology
  • Genetics
  • Virology

Background:

  • Bacteriophage lambda site-specific recombination involves integration and excision.
  • Lambda integrase (Int) mediates both integration and excision reactions.
  • Excisionase (Xis) assists Int in DNA binding and bending for efficient excision.

Purpose of the Study:

  • To investigate the protein-protein interactions between lambda integrase (Int) and excisionase (Xis).
  • To identify the specific domains of Int and Xis involved in their interaction during recombination.

Main Methods:

  • Gel mobility shift assays were employed to analyze DNA-protein and protein-protein interactions.
  • The study focused on the interaction between Int and Xis in the context of lambda recombination.

Related Experiment Videos

Main Results:

  • The amino-terminal domain of Int was found to maintain cooperative interactions with Xis.
  • This interaction suggests a direct role for the amino-terminal DNA-binding domain of Int in binding Xis.

Conclusions:

  • The carboxyl-terminal alpha-helix of Xis is not the sole interaction site with Int.
  • The amino-terminal domain of Int directly interacts with Xis, contributing to the formation of the excisive nucleoprotein complex.