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Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice
Published on: August 12, 2011
Chicken cells--oncogene transformation, immortalization and more
1Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 166 37 Prague 6, Czech Republic. plachy@img.cas.cz
Folia Biologica
|August 27, 2002
Summary
Domestic chickens and their cells are valuable in experimental biology, particularly for studying viral oncology and cell transformation. Recent advances with chicken cell lines offer new research avenues.
Area of Science:
- Experimental biology
- Cell biology
- Oncology
Background:
- Domestic chickens and chicken cells are established models in experimental biology, especially for retrovirology and oncology.
- Chicken cells possess a long genetic stability, advantageous for certain research but limiting for immortalization.
- Historically, the first viral tumor etiology and in vitro oncogenic transformation measurements were developed using chickens.
Purpose of the Study:
- To review traditional achievements using the chicken model in experimental biology.
- To highlight new applications of chicken cells, particularly in cell transformation and immortalization research.
- To discuss the utility of newly established continuous chicken cell lines.
Main Methods:
- Review of historical and recent scientific literature on chicken models.
- Analysis of the properties of chicken cells, including lifespan and genetic stability.
- Evaluation of the application of specific chicken cell lines, such as DT40, in gene disruption studies.
Main Results:
- Chicken cells exhibit long-term genetic stability, similar to human cells.
- The DT40 chicken B cell line demonstrates a high frequency of gene targeting, useful for gene disruption.
- New continuous chicken cell lines have overcome limitations in cell immortalization.
Conclusions:
- The chicken model continues to be a significant tool in experimental biology and oncology.
- Chicken cells, especially novel cell lines, offer unique advantages for studying cell transformation, immortalization, and gene function.
- The chicken system provides a powerful platform for advancing our understanding of cancer biology and genetic manipulation.
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