Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Type I-interferon signalling in fish.

Bertrand Collet1, Christopher J Secombes

  • 1Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen AB24 2TZ, U.K.

Fish & Shellfish Immunology
|August 27, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of cell lines persistently infected with infectious pancreatic necrosis virus.

The Journal of general virology·2026
Same author

Evolutionary Conservation, Expansion and Diversification of Interferon stimulated genes (ISG) in Vertebrates: insights from fish.

Biomedical journal·2026
Same author

Erratum: N1MΨU-modified mRNA vaccines break the mold in fish by enhancing innate immune activation.

Molecular therapy. Nucleic acids·2026
Same author

Development and Validation of a Multiplex RT-PCR Assay for the Simultaneous Detection of Three Major Viral Pathogens in Salmonids: IHNV, ISAV and SAV.

Journal of fish diseases·2026
Same author

N1MΨU-modified mRNA vaccines break the mold in fish by enhancing innate immune activation.

Molecular therapy. Nucleic acids·2026
Same author

Toll-like Receptor 3 is Required for Sensing Extracellular Double Stranded RNA in Salmonid Cells.

Marine biotechnology (New York, N.Y.)·2026
Same journal

Common carp USP30s restrict SVCV replication by attenuating mitophagy.

Fish & shellfish immunology·2026
Same journal

Epigallocatechin-3-gallate attenuates the immunotoxicity of sulfamethoxazole and reduces its residues in crayfish via phagocytosis.

Fish & shellfish immunology·2026
Same journal

LvBlimp1 and LvHes1 coregulate the function of macrophage-like phagocytes in shrimp hemolymph.

Fish & shellfish immunology·2026
Same journal

Functional remodeling of IgM<sup>+</sup> B cells in Streptococcus agalactiae-resistant Nile tilapia.

Fish & shellfish immunology·2026
Same journal

Generation and application of monoclonal antibodies against CD4-1 and CD8α for characterizing T cell subsets in grass carp (Ctenopharyngodon idella).

Fish & shellfish immunology·2026
Same journal

Temperature during vaccination shapes antibody responses and protection against infectious salmon anemia in Atlantic salmon under different prime-boost vaccine strategies.

Fish & shellfish immunology·2026
See all related articles

This study identifies the regulatory sequence of the trout interferon regulatory factor-1 (IRF-1) gene, crucial for antiviral responses. The findings reveal its promoter structure and inducibility by viral RNA, advancing our understanding of fish innate immunity.

Area of Science:

  • Immunology
  • Genetics
  • Molecular Biology

Background:

  • Type I interferon (IFN) signaling is vital for antiviral defense, involving the Jak-Stat pathway and transcription factors like interferon regulatory factor (IRF)-1 and -2.
  • IRF-1 and -2 regulate IFN-induced genes, with IRF-1 stimulating and IRF-2 repressing gene expression.
  • Understanding the genetic regulation of IFN-induced genes in fish is crucial for aquaculture and disease resistance.

Purpose of the Study:

  • To clone and sequence the regulatory region of the trout IRF-1 gene.
  • To analyze the promoter structure of the trout IRF-1 gene for elements characteristic of IFN-induced promoters.
  • To investigate the expression and inducibility of trout IRF-1 in response to viral stimuli.

Main Methods:

  • Gene walking using trout genomic DNA to isolate the IRF-1 gene's regulatory sequence.

Related Experiment Videos

  • Sequence analysis of the 5' flanking region to identify promoter elements (TATA box, NF kappa B, GAAA motifs).
  • Transient transfection assays in rainbow trout gonad (RTG) cells using a luciferase reporter construct to assess promoter activity and induction by poly I:C (a viral RNA mimic) and LPS.

    • Main Results:

    • The 1 Kb 5' flanking region of the trout IRF-1 gene was successfully cloned and sequenced.
    • Sequence analysis revealed a promoter structure typical for IFN-induced genes, including a TATA box, NF kappa B site, and multiple GAAA motifs, but lacking a complete ISRE.
    • Trout IRF-1 was constitutively expressed in RTG cells and upregulated by poly I:C but not LPS, confirming its role in antiviral response.
    • The identified 5' flanking region was sufficient to drive luciferase expression and was inducible by dsRNA (poly I:C) in RTG cells.

    Conclusions:

    • The cloned 5' flanking region of the trout IRF-1 gene contains functional promoter elements necessary for its expression and induction by viral stimuli.
    • These findings provide insights into the molecular mechanisms of interferon signaling and antiviral immunity in fish.
    • The study highlights the conserved nature of IFN-inducible promoter elements across vertebrates.