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Updated: Sep 29, 2026
Semi-Minimal Invasive Method to Induce Myocardial Infarction in Rats and the Assessment of Cardiac Function by an Isolated Working Heart System
Published on: June 11, 2020
[Experimental study on effects of self-made miniature axial flow blood pump as left ventricle assistance for
Xiaotong Hou1, Yanqing Sun, Changyan Lin
1Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital University of Medical Sciences, Beijing 100029, China.
Objective:
To evaluate the effects of self-made miniature axial flow blood pump as left ventricle assistance for postischemic heart.
Methods:
Sixteen adult healthy sheep were divided into two groups of 8 sheep. After acute left heart failure was induced by selective ligation of the left anterior descending branch of coronary artery for 1.5 hours and ligation of the left circumflex branch for half an hour, one hour of reperfusion with assistance by self-made blood pump was offered in experimental group and no mechanical assistance was given to the control group. The sheep were killed after the experiment and their hearts were taken to be observed. During the experiment, the changes in hemodynamic and hemologic indices were compared between the two groups to assess the effects of left heart assistance on hemodynamics and blood by self-made blood pump.
Results:
After 1 hour of assistance by self-made blood pump, experimental group, in comparison with the control group, showed a significant (P < 0.05) increase in total cardial output (3.11 +/- 0.3 vs 2.37 +/- 0.3 L/min), mean aortic pressure (73 +/- 5.4 vs 66 +/- 3.1 mm Hg), and left ventricular Dp/Dt(max) (1 415 +/- 2 549 vs 986 +/- 8 mm Hg/sec); and a significant decrease in left ventricular end diastolic pressure (11.8 +/- 2.1 vs 13.5 +/- 1.4 mm Hg) and left atrial pressure (14.1 +/- 1.6 vs 16.0 +/- 1.6 mm Hg) (all P < 0.05). There was no significant difference in plasma free hemoglobin (12.4 +/- 3.1 vs 13.8 +/- 4.7 mg/dl) and fibrinogen (0.32 +/- 0.12 vs 0.42 +/- 0.12 g/dl) between these two groups.
Conclusion:
1, Animal model for acute left heart failure can be achieved by selective ligation of coronary arteries and satisfactorily used in left ventricular assist device assessment. 2. Self-made blood pump can be used in assistance for failure heart without serious event.
